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Fig. 3 | Mobile DNA

Fig. 3

From: Ribosomal protein and biogenesis factors affect multiple steps during movement of the Saccharomyces cerevisiae Ty1 retrotransposon

Fig. 3

Ty1 Gag protein expression in various mutants defective in ribosomal biogenesis. Total cell protein was extracted from wild type (WT) and mutant strains, and subjected to Western blot analysis (50 μg/lane) using anti-VLP (upper panel) and control Hts1p (histidyl-tRNA synthetase; lower panel) antibodies. The Gag precursor (p49) and altered forms of Gag (Gag), which co-migrate under these electrophoretic conditions, and mature (p45) protein are indicated. Hts1p was used as a loading control. The extent of antibody reaction to both p49/Gag and p45 species of Gag, quantified using ImageJ, is shown beneath each cofactor mutant strain expressed as a ratio to that of the wild type strain. LSU = large (60S) ribosomal subunit; SSU = small (40S) ribosomal subunit

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