- Open Access
Erratum to: Reliable transgene-independent method for determining sleeping beauty transposon copy numbers
Mobile DNAvolume 4, Article number: 11 (2013)
The original article was published in Mobile DNA 2011 2:5
After the publication of our article , the authors noticed two typing errors in the ’Methods’ part of the original article:
in Table two, the correct sequence of RPPH1 / Probe is 2 nucleotides longer at the 3’ end, so it is: CACTCCCATGTCCCTT ;
in the penultimate sentence of the 'Quantitative real-time PCR' section, the concentrations of the primers and the probe were switched so the correct sentence should be: "Final concentrations of primers and probes were 900 and 250 nM, respectively."
The authors apologize for these unintended mistakes in the text.
Kolacsek O, Krízsik V, Schamberger A, Erdei Z, Apáti Á, Várady G, Mátés L, Izsvák Z, Ivics Z, Sarkadi B, Orbán TI: Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers. Mob DNA. 2011, 2: 5-10.1186/1759-8753-2-5.
The authors declare that they have no competing interests.
OK established the HEK clones, OK and VK optimized the real-time PCR and performed copy number measurements, AS, ZE and ÁA established the HUES9 clones, GV helped in FACS measurements, LM measured copy numbers in HeLa clones, ZsI and ZI gave technical help and advices with the SB transposon work, BS provided financial support and discussed the data and TIO designed the overall strategy, analyzed the data and wrote the paper. All authors read and approved the final manuscript.
The online version of the original article can be found at 10.1186/1759-8753-2-5