Universal platform for quantitative analysis of DNA transposition

Table 1 Colony phenotypes and papillation characteristics in different E. coli strains

Strain	Colony size^a	Adjustabilitywith arabinose	Maximum frequencyof transposition^b	Distribution ofpapillae on colonies	Papillae number dependency on the number of growing colonies per plate^c
DH10B	Large	Limited	High (up to ~100 papillae)	Centered in the middle	Somewhat dependent
DH5α	Large	Good	Highest (up to ~300 papillae)	Even	Largely independent
JM109	Medium	Good	Intermediate (up to ~40 papillae)	Even	ND^d
HT321	Large	Moderate	Low (up to ~10 papillae)	Even	ND

^aLarge colony size: up to ~5 mm of diameter. Medium colony size: up to ~3 mm of diameter.
^bThe maximum frequency of transposition is specified in this table as the number of papillae per colony grown at 30°C for 115 h on a standard papillation medium containing 0.1% arabinose.
^cSee Additional file 2.
^dND, not determined.

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