Fig. 2
From: CRISPR-TE: a web-based tool to generate single guide RNAs targeting transposable elements
Screenshot of The CRISPR-TE Web Tool Interface. A The CRISPR-TE homepage, which requires three types of input: (i) Design purpose, (ii) Genome assembly, and (iii) Target TE copy ID or genomic coordinates. B Tool is provided for querying individual TE copy IDs and their genomic coordinates. C After submitting, CRISPR-TE displays all possible sgRNAs along with detailed information, including sgRNA sequence, coordinates, GC content, mismatches, on-target score, and off-target score. D CRISPR-TE enables users to examine the locations of sgRNAs on the genome, alongside other genomic features, by clicking on individual sgRNAs. The pie chart on the left illustrates the proportions of target sites with various mismatch counts. A list of all off-target sgRNA, including their sequences, genomic coordinates, and associated genetic element classes, is shown on the right. E The results for sgRNA combinations targeting TE subfamilies are presented. This includes the sgRNA sequences, the number of on-target sites, the on-target percentage for the queried TE subfamily, the sgRNA combination coverage, and off-targets on TEs and other genetic element classes