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Fig. 8 | Mobile DNA

Fig. 8

From: Mobile group I introns at nuclear rDNA position L2066 harbor sense and antisense homing endonuclease genes intervened by spliceosomal introns

Fig. 8

Mapping of I-DsqI homing endonuclease cleavage site. The cleavage site was mapped using primer extension (PE) and Sanger sequencing in parallel when using specific primers for analysing both strands of the target DNA. Products were separated on a 5% denaturing polyacrylamide gel. Below: summary of cleavage site mapping showing a pentanucleotide 3′ overhang. Vertical arrow indicates site for intron insertion

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Mobile DNA

ISSN: 1759-8753