Fig. 3

Differential contribution of monomer 2, monomer 1 and tether to overall promoter activity in NIH/3T3 cells. Normalized promoter activity of individual 5’UTR domains for subfamily Tf_I (A), A_I (C), and Gf_I (E). Sequence organization of the promoters is illustrated on the left side. The length of M2, M1, and tether for each promoter is annotated (in base pairs). The dashed line represents domain(s) that were removed in reference to the two-monomer 5’UTR sequence (M2-M1-T). The tether was tested in both sense (T) and antisense (T_AS) orientation. A short version of Gf_I tether was additionally included (T249 and T249_AS) in panel E. The x-axis indicates the normalized promoter activity (i.e., the Fluc/Rluc ratio of a control no-promoter vector, pLK037, was set to 1). Note a broken x-axis was used to highlight the wide range of promoter activities. On the right hand are 2-D representations of the promoter data for subfamily Tf_I (B), A_I (D), and Gf_I (F), corresponding to panel A, panel C, and panel E, respectively. Each domain tested is represented by a filled box. The domains are arranged in the order of M2, M1, and tether from left to right. The height of the box corresponds to the normalized promoter activity (to scale). A scale is shown in panel F; its height corresponds to a normalized promoter activity of 100. The hatched lines represent the missing M1 domain in the M2-T promoter construct