Fig. 4
From: Unbiased proteomic mapping of the LINE-1 promoter using CRISPR Cas9
DUSP1 modulates LINE-1 mRNA expression. A qPCR of LINE-1 mRNA levels in E006AA-hT cells expressing shRNA to DUSP1 or non-targeting control (NS). B qPCR of relative LINE-1 levels in E006AA-hT cells that were treated with vehicle (DMSO) or DUSP1 inhibitor BCI. C qPCR of relative LINE-1 mRNA levels in E006AA-hT or LNCaP cells overexpressing vector only (VO) or DUSP1. D qPCR of DUSP1 mRNA levels in panel of prostate cancer cell lines (including E006AA-hT renal cell carcinoma line). E qPCR of relative LINE-1 mRNA levels (left) and western blots of LINE-1 ORF1p, DUSP1, and hsp90 (loading control) protein levels (right) in PC3 cells that were treated with siScramble or siDUSP1 siRNA. ORF1 protein levels relative to hsp90 were quantified, siScramble was set to 1