Fig. 1

Sequence alignment of the CRDs of PBLE transposases and PGBD domesticated proteins. All accession numbers can be found in File S1. PBLE transposases: Ago (Aphis gossypii); Bmo (Bombyx mori); Cag (Ctenoplusia agnate); Har (Helicoverpa armigera); Hvi (Heliothis virescens); PB-Tni (Trichoplusia ni); Mlu (PiggyBat from Myotis lucifugus); PLE-wu (Spodoptera frugiperda). Domesticated PGBD transposases: Oni (Oreochromis niloticus); Pny (Pundamilia nyererei); Lia5, Tpb1, Tpb2, Tpb6 and Tpb7 (Tetrahymena thermophila); Pgm, PgmL1, PgmL2, PgmL3a/b/c, PgmL4a/b, PgmL5a/b (Paramecium tetraurelia); Tru (Takifugu rubripes); Pgbd2, Pgbd3 and Pgbd4 (Homo sapiens). In the sequence of Pgm(692–768), the coordinates of histidine residues are indicated, with His701 and His738 in red. In all sequences, the upstream histidine corresponding to Pgm His701 is highlighted in grey, when present. Conserved residues specific for the PB CRD are highlighted in yellow, those specific for the Pgm CRD are highlighted in green. For each peptide, isoelectric points (on the right: in blue or red for basic or acidic peptides, respectively) were calculated for the sequences highlighted in orange, using the ExPASy Compute pI/MW tool (https://web.expasy.org/compute_pi/)