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Table 2 Top-12 L. lactis IL1403 mutants with increased RTP frequencies

From: Methylation of rRNA as a host defense against rampant group II intron retrotransposition

  ISS1 Insertion Position Gene Library tag Gene product Protein Accession Relative RTP Frequency COG Category
1 1,959,088 rsmB PL1B3 16S rRNA cytosine methyltransferase (m5C) at position 967 WP_010906185.1 1.3+/−0.7 J, K
2 108,704 spoT PL5A3 Bifunctional (p)ppGpp synthetase/guanosine-3′5’-bis(diphosphate) 3′ - pyrophosphohydrolase WP_010905119.1 4.4+/−1.6 K, T
3 1,785,586 coiA PL5A12 Competence protein, contains a predicted nuclease domain WP_010906110.1 5.7+/−3.2 R
4 623,717 rpoE PL6A8 DNA-directed RNA polymerase subunit delta WP_003129551.1 9.3+/−6.6 K
5 105,498 rsmE PL6B3 16S rRNA uracil methyltransferase (m3U) at position 1498 WP_010905117.1 1.7+/−1.3 J
6 1,785,385 coiA PL6F9 Competence protein, contains a predicted nuclease domain WP_010906110.1 6.8+/−3.6 R
7 2,202,641 rlmH PL7B7 23S rRNA pseudouridine methyltransferase (m3Ψ) at position 1915 WP_003130585.1 20.1+/−1.5 J
8 1,235,064 hisH PL7D6 Imidazole glycerol phosphate synthase subunit WP_010905832.1 81.3+/−75.3 E
9 453,621 recT PL7E1 Recombinational DNA repair protein WP_010905348.1 10.5+/−4.3 L
10 62 oriC PL8C4 Origin of replication, intergenic   5.3+/−5.2  
11 315,800 ybaK PL9C11 Aminoacyl-tRNA deacylase WP_003131680.1 3.8+/−3.4 J
12 1,785,006 coiA PL11D6 Competence protein, contains a predicted nuclease domain WP_010906110.1 31.1+/−34.4 R