Table 2 Top-12 L. lactis IL1403 mutants with increased RTP frequencies
From: Methylation of rRNA as a host defense against rampant group II intron retrotransposition
| Â | ISS1 Insertion Position | Gene | Library tag | Gene product | Protein Accession | Relative RTP Frequency | COG Category |
|---|---|---|---|---|---|---|---|
1 | 1,959,088 | rsmB | PL1B3 | 16S rRNA cytosine methyltransferase (m5C) at position 967 | WP_010906185.1 | 1.3+/−0.7 | J, K |
2 | 108,704 | spoT | PL5A3 | Bifunctional (p)ppGpp synthetase/guanosine-3′5’-bis(diphosphate) 3′ - pyrophosphohydrolase | WP_010905119.1 | 4.4+/−1.6 | K, T |
3 | 1,785,586 | coiA | PL5A12 | Competence protein, contains a predicted nuclease domain | WP_010906110.1 | 5.7+/−3.2 | R |
4 | 623,717 | rpoE | PL6A8 | DNA-directed RNA polymerase subunit delta | WP_003129551.1 | 9.3+/−6.6 | K |
5 | 105,498 | rsmE | PL6B3 | 16S rRNA uracil methyltransferase (m3U) at position 1498 | WP_010905117.1 | 1.7+/−1.3 | J |
6 | 1,785,385 | coiA | PL6F9 | Competence protein, contains a predicted nuclease domain | WP_010906110.1 | 6.8+/−3.6 | R |
7 | 2,202,641 | rlmH | PL7B7 | 23S rRNA pseudouridine methyltransferase (m3Ψ) at position 1915 | WP_003130585.1 | 20.1+/−1.5 | J |
8 | 1,235,064 | hisH | PL7D6 | Imidazole glycerol phosphate synthase subunit | WP_010905832.1 | 81.3+/−75.3 | E |
9 | 453,621 | recT | PL7E1 | Recombinational DNA repair protein | WP_010905348.1 | 10.5+/−4.3 | L |
10 | 62 | oriC | PL8C4 | Origin of replication, intergenic |  | 5.3+/−5.2 |  |
11 | 315,800 | ybaK | PL9C11 | Aminoacyl-tRNA deacylase | WP_003131680.1 | 3.8+/−3.4 | J |
12 | 1,785,006 | coiA | PL11D6 | Competence protein, contains a predicted nuclease domain | WP_010906110.1 | 31.1+/−34.4 | R |