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Fig. 3 | Mobile DNA

Fig. 3

From: LUMI-PCR: an Illumina platform ligation-mediated PCR protocol for integration site cloning, provides molecular quantitation of integration sites

Fig. 3

The most-clonal integrations are found reproducibly in all replicate libraries. a) A four-way Venn diagram illustrates the number of integrations that are found reproducibly in 1, 2, 3 and 4 replicate libraries. The majority of single fragment/subclonal integrations are only found in one library whereas the most-clonal integrations are found in all four libraries. The clonality values b) and normalized clonality values c) of all integrations were compared for integrations that were found in 1, 2, 3 and 4 replicate libraries. The set of mutations present in only one of the four libraries had substantially lower median clonality/normalized clonality values than those inserts found in more than one library. Although the vast majority of subclonal mutations were found in only one library, a fraction are also found in more than one library. All integrations with clonality > 0.01 and normalized clonality > 0.1 were found present in all four libraries

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ISSN: 1759-8753