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Table 1 Transposition frequencies of MBP-tagged or untagged Hsmar1 transposase

From: Compensating for over-production inhibition of the Hsmar1 transposon in Escherichia coli using a series of constitutive promoters

Construct

Transposition frequency

Ptac MBP-Hsmar1 transposase

1.99 (±0.43) × 10−6

Ptac Hsmar1 transposase

2.58 (±0.02) × 10− 5

  1. The bacterial mating-out assays have been performed with the RC5097 strain with the pRC880 or pRC1721 vectors and in absence of lactose and IPTG. Transposition frequencies are the average of three independent experiments ± standard error of the mean
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Mobile DNA

ISSN: 1759-8753