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Table 1 Whole-genome sequencing of 23 M. hominis strains

From: Characterisation of mobile genetic elements in Mycoplasma hominis with the description of ICEHo-II, a variant mycoplasma integrative and conjugative element

Strain

Short-read sequencing

Long-read sequencing

Protocol

Generated data (Mb)

Est. coverage (X)

Technology

Kit

Generated data (Mb)

Est. coverage (X)

Median read length/kb

FBG

2 × 300

1612

2067

Nanopore

SQK-RAD003

3542

4541

2.5

8958

2 × 300

157

231

Nanopore

SQK-RAD003

657

966

4.5

2539

2 × 300

2344

3119

Nanopore

SQK-LSK108

384

511

12.6

A136

2 × 300

240

344

Nanopore

EXP-NBD103 + SQK-LSK108

707

1016

13.6

SP2565

2 × 300

205

287

Nanopore

1092

1533

10.5

475

2 × 300

184

257

Nanopore

638

889

5.3

SS10

2 × 300

1648

2353

Nanopore

756

1080

5.7

SS25

2 × 300

1865

2772

Nanopore

732

1088

8.3

VO31120

2 × 250

89

130

Nanopore

728

1069

6.3

SP10291

2 × 250

80

106

Nanopore

562

749

4.4

SP3615

2 × 250

99

138

PacBio

SMRTbell Template Prep Kit 1.0 + Sequel Binding and Internal Control Kit 2.1

7447

10,401

2.9

727 J

   

Nanopore

EXP-NBD103 + SQK-LSK108

17

21

5.2

942 J

   

Nanopore

38

47

44.1

2740

   

Nanopore

15

16

4.1

7388VA

   

Nanopore

39

47

41.2

7447VA

   

Nanopore

59

66

1.7

10936VA

   

Nanopore

53

66

3.0

12256 U

   

Nanopore

35

42

14.5

14352VA

   

Nanopore

37

47

32.3

16753

   

Nanopore

7

9

4.4

18847

   

Nanopore

38

49

45.7

19791

   

Nanopore

44

52

4.6

21127VA

   

Nanopore

38

44

42.4