Table 3 ME-Scan amplification conditions
| First amplification | Second amplification | |||||
|---|---|---|---|---|---|---|
| AluYb (5 cycles) | L1HS* | SVA (10 cycles) | AluYb (20 cycles) | L1HS (12 cycles) | SVA (12 cycles) | |
| PCR grade water | As needed | As needed | ||||
| 2X KAPA HiFi HS RM | 25 μl | 25 μl | 37.5 μl | |||
| Adapter primer (P7)+ | 2.5 μl | 2.5 μl | 3.75 μl | |||
| ME-specific primer + | 2.5 μl | 2.5 μl | 3.75 μl | |||
| DNA | 360 ng | 100 ng | 200 ng | 16 μl | 2 μl | 24 μl |
| Total | 50 μl | 50 μl | 75 μl | |||