Table 3 ME-Scan amplification conditions
| Â | First amplification | Second amplification | ||||
|---|---|---|---|---|---|---|
|  | AluYb (5 cycles) | L1HS* | SVA (10 cycles) | AluYb (20 cycles) | L1HS (12 cycles) | SVA (12 cycles) |
PCR grade water | As needed | As needed | ||||
2X KAPA HiFi HS RM | 25 μl | 25 μl | 37.5 μl | |||
Adapter primer (P7)+ | 2.5 μl | 2.5 μl | 3.75 μl | |||
ME-specific primer + | 2.5 μl | 2.5 μl | 3.75 μl | |||
DNA | 360 ng | 100 ng | 200 ng | 16 μl | 2 μl | 24 μl |
Total | 50 μl | 50 μl | 75 μl | |||