Nuclear export signal (NES) of transposases affects the transposition activity of mariner-like elements Ppmar1 and Ppmar2 of moso bamboo

Table 1 Amino acid mutations in NES domains of Ppmar1-NES and Ppmar2-NES transposases of moso bamboo

Names of NES	NES type	Sequence	Mutated site	Approximate NES score
Ppmar1-NES-0	Wild type NES	KQRLEREDRLPLQIP	–	0.9
Ppmar1-NES-1	Strong NES	KLRLEREDRLPLQIP	Q → L	1
Ppmar1-NES-2	Weak NES	KQRLEREDRAPAQIP	L → A; L → A	0
Ppmar1-NES-3	Intermediate NES	KQRLEREDRAEIQIP	L → A; P → E; L → I	0.5
Ppmar2-NES-0	Wild type NES	RNGVLSIRLQCDL	–	0.85
Ppmar2-NES-1	Strong NES	RLGVLSIRLQCDL	N → L	1
Ppmar2-NES-2	Weak NES	RNGVLSARLQCDL	I → A	0
Ppmar2-NES-3	Intermediate NES	RNGVLLIRLQCDL	S → L	0.5

The bold red letters stand for mutated amino acids
Letters on the left- and right-hand sides of the arrows indicate the wild type and the mutated amino acid, respectively. The NES types were identified based on the NES scores
Q glutamine, L leucine, A alanine, P proline, E glutamic acid, N asparagine, I isoleucine, S serine

ISSN: 1759-8753