Table 1 R2Ol retrotransposed into the zebrafish 28S rDNA and transmitted to next generation
From: Targeted gene knockin in zebrafish using the 28S rDNA-specific non-LTR-retrotransposon R2Ol
mRNA constructs | F0 retrotrans-position | Founder number | Founder (F0) | Germline mosaicism | Insertion copies | F1 name | F2 GFP+ | ||
|---|---|---|---|---|---|---|---|---|---|
adult (fin) | embryo | total | |||||||
R2Ol-r100-v | 53/55 (96%) | 8/16 (50%) | R2f-2 | 8/25 | 4/30 | 12/55 (22%) | 3 | – | – |
R2f-5 | – | 4/30 | 4/30 (13%) | – | – | – | |||
R2f-6 | 2/8 | – | 2/8 (25%) | – | – | – | |||
R2f-7 | 4/33 | 5/30 | 9/63 (14%) | – | – | – | |||
R2f-9 | 2/10 | – | 2/10 (20%) | 2 | – | – | |||
R2f-10 | 2/3 | 18/39 | 20/42 (48%) | 1 | – | – | |||
R2f-11 | – | – | – | – | – | – | |||
R2f-12 | – | – | – | – | – | – | |||
r105-R2Ol-EF1-EGFP(F)-r10 | 17/20 (85%) | 2/27 (7.4%) | GFm-6 (truncated) | – | – | – | – | – | – |
GFm-13 (truncated) | – | – | – | – | – | – | |||
r105-R2Ol-EF1-EGFP(R)-r10 | 21/22 (95%) | 4/25 (16%) | GRm-1 | 1/42 | 5/30 | 6/72 (8.3%) | 1 | GRm-1-32 | Â |
GRm-12 (truncated) | – | – | – | – | – | – | |||
GRm-14 | 3/7 | 17/30 | 20/37 (54%) | 1 | GRm-14-1 | 1/51 | |||
GRm-14-7 | 2/124, 1/66 | ||||||||
GRf-2 | 3/21 | 2/30 | 5/51 (9.8%) | 1 | – | – | |||
r105-R2Ol-hsp-GAL4R-r10 | 36/58 (62%) | 1/15 (6.6%) | G4Rf-201 | 5/11 | 8/30 | 13/41 (32%) | 1 | G4Rf-201 | 15/29 |
R2OlΔ3′ UTR-v + EGFP(R)-3′ UTR-r10 | 3/44 (6.8%) | – | – | – | – | – | – | – | – |