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Table 1 R2Ol retrotransposed into the zebrafish 28S rDNA and transmitted to next generation

From: Targeted gene knockin in zebrafish using the 28S rDNA-specific non-LTR-retrotransposon R2Ol

mRNA constructs F0 retrotrans-position Founder number Founder (F0) Germline mosaicism Insertion copies F1 name F2 GFP+
adult (fin) embryo total
R2Ol-r100-v 53/55 (96%) 8/16 (50%) R2f-2 8/25 4/30 12/55 (22%) 3
R2f-5 4/30 4/30 (13%)
R2f-6 2/8 2/8 (25%)
R2f-7 4/33 5/30 9/63 (14%)
R2f-9 2/10 2/10 (20%) 2
R2f-10 2/3 18/39 20/42 (48%) 1
R2f-11
R2f-12
r105-R2Ol-EF1-EGFP(F)-r10 17/20 (85%) 2/27 (7.4%) GFm-6 (truncated)
GFm-13 (truncated)
r105-R2Ol-EF1-EGFP(R)-r10 21/22 (95%) 4/25 (16%) GRm-1 1/42 5/30 6/72 (8.3%) 1 GRm-1-32  
GRm-12 (truncated)
GRm-14 3/7 17/30 20/37 (54%) 1 GRm-14-1 1/51
GRm-14-7 2/124, 1/66
GRf-2 3/21 2/30 5/51 (9.8%) 1
r105-R2Ol-hsp-GAL4R-r10 36/58 (62%) 1/15 (6.6%) G4Rf-201 5/11 8/30 13/41 (32%) 1 G4Rf-201 15/29
R2OlΔ3′ UTR-v + EGFP(R)-3′ UTR-r10 3/44 (6.8%)
  1. The retrotransposition efficiency (%) = transposed embryo no. / analyzed embryo no. × 100