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Fig. 6 | Mobile DNA

Fig. 6

From: Characterization of a relaxase belonging to the MOBT family, a widespread family in Firmicutes mediating the transfer of ICEs

Fig. 6

RelSt3 activity in the presence of various divalent cations. a RelSt3 relaxation activity in the presence of various divalent cations. 0, 0.5 or 5 μg of RelSt3 (as indicated) were incubated for 20 min at 30 °C with pBR322-oriT, without or with 5 mM of the indicated cation (Ca2+, Zn2+, Ni2+, Co2+, Mg2+ or Mn2+). b RelSt3 relaxation activity in the presence of various concentration of Mn2+. 5 μg of RelSt3 protein were incubated for 20 min at 30 °C with plasmidic DNA with 0.01, 0.05, 0.1, 0.25, 0.5, 2, 5 or 10 mM of Mn2+

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Mobile DNA

ISSN: 1759-8753