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Fig. 1 | Mobile DNA

Fig. 1

From: Transcriptionally promiscuous “blurry” promoters in Tc1/mariner transposons allow transcription in distantly related genomes

Fig. 1

Overall scheme of the expression cassettes tested in this study. The tested sequences of Tc1-like, mariner-like and hobo elements (a, b and c) are divided into a blue region containing the transposase binding sites (depicted as dashed boxes) and sequences situated between the upstream TIRs and the transposase ORFs in these transposons (green region). These fragments are directly fused to a luciferase reporter gene (yellow). The LTR-retrotransposon sequences (d) are depicted with their canonical U3-R-U5 structure (more details are given in the main text). The organization of the negative (e) and positive (f) construct are also reported. Drawings are not in scale

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