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Fig. 2 | Mobile DNA

Fig. 2

From: The toxic guardians — multiple toxin-antitoxin systems provide stability, avoid deletions and maintain virulence genes of Pseudomonas syringae virulence plasmids

Fig. 2

Stability of constructs containing the native RepA-PFP and RepJ replicons from pPsv48C, and their chimeras. a Fragments of the RepA-PFP (black) or RepJ (white) replicons, and their chimeras, were cloned at the indicated positions into pKMAG; small and large arrows represent the putative leader peptide and the replication initiator genes, respectively. TT, T4 transcription terminator; MCS, multiple cloning site; kan, kanamycin resistance gene. b Percentage (mean ± sd) of P. syringae pv. syringae B728a cells (dark grey) or of P. syringae pv. savastanoi UPN912 cells (light grey) retaining each of the constructs of panel a means with different letters are significantly different (two-way ANOVA and Duncan’s multiple range test; p < 0.05). Experiments were repeated three times, each with three replicates

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