Table 5 PCR master mix formulas
PCR master mix | Volume (Ī¼L) | |
|---|---|---|
1x | 8x | |
Molecular grade H2O | 32.55 | 260.4 |
10x Ex Taq buffer | 5.0 | 40 |
dNTP mixture (2.5āmM each) | 4.0 | 32 |
Specific L1 Primer (100āĪ¼M) | 0.1 | 0.8 |
Vectorette Primer (100āĪ¼M) | 0.1 | 0.8 |
Ex Taq HS polymerase | 0.25 | 2.0 |