Fig. 5
From: The SAMHD1-mediated block of LINE-1 retroelements is regulated by phosphorylation
The dNTP hydrolase activity of SAMHD1 is not regulated by phosphorylation at T592 in 293T cells. 293T shC and shSAMHD1 cells were transfected with empty vector (pcDNA), SAMHD1 wt, non-phosphorylated SAMHD1 T592A, the phosphomimetic mutant T592D, the HD domain mutant D207N, the RNase mutant Q548A, the allosteric site mutant D137A, or the oligomerization-defective mutant L428S/Y432S. Two days posttransfection, cells were lysed and intracellular dNTP concentrations were determined by HPLC analysis (a) or single dNTP incorporation assays (b) The average of triplicate analysis for dATP (μM) is shown. The analysis for the other dNTPs yielded similar results. Error bars indicate the standard deviation. Statistical analysis was done using one way ANOVA followed by Tukey’s multiple comparison test. ns, not significant