Fig. 5
From: Long interspersed nuclear element-1 expression and retrotransposition in prostate cancer cells
Retrotransposition Assay. The retrotransposition plasmid (pPM016) was transfected into cells. LINE-1 mRNA is expressed and processed, removing the γ globin intron from GFP during RNA splicing. ORF1p and ORF2p are translated and bind to LINE-1 mRNA, forming ribonucleoprotein (RNP) particles. The RNP enters the nucleus, where LINE-1 mRNA is reverse transcribed into host DNA by Target Primed Reverse Transcription (TPRT). After retrotransposition, cells expressing functional GFP are quantified by flow cytometry