Fig. 7

Target site preferences of high-copy Class 2 transposons from barley. For the plots, the 30 bp flanking complete elements (i.e., not truncated) on both sides were collected. Then the different nucleotides at each position were counted across across all insertion sites of a given TE type. The x-axis is the bp position relative to the TE insertion site, while the y-axis shows the relative nucleotide composition for each position. a Mariner elements have a strong preference for A/T dinucleotides 2 and 5 bp away from the insertion site, while (b) Harbinger elements almost invariable prefer 3 bp A/T -rich motifs. c Interestingly, Helitrons have a preference for an asymmetric target, strongly preferring an AAA motif 8 bp downstream of the insertion site