Fig. 2

Screenshot of the result display. In the figure, we show the mapping of the insertion coordinate when using a query sequence derived by iPCR from a P{lacW} hit affecting lama gene from D. melanogaster. The red rectangles stand for the transposon fragments, the blue ones represent the genomic sequence and the green ones stand for annotations of lama gene and of 3′ TIR of P{lacW}. Herein, the TGN is the C nucleotide located just next to the terminal coordinate 10691 of P{lacW}, which is also a C nucleotide. Hence, the insertion coordinate explicitly reported by Genome ARTIST with blue digits is 5348435. The genomic coordinate 5348475 is the one bordering the GATC restriction site of Sau3A1 used in the iPCR procedure. Since the restriction site belongs both to the transposon and to the local genomic region, it is arbitrarily allocated to the transposon sequence. Herein, we used a query sequence which contains the two transposon fragments encompassing the genomic sub-sequence