Fig. 2

Transposition assays in yeast with altered DNA repair potentials. ADE2 revertant frequencies for the mPing and 14T32-T7 elements in yeast strains with different DNA repair mechanisms available for excision site repair (a). JIM17 repairs by NHEJ, CB101 is capable of both HR and NHEJ, and DG21B9 can only repair by HR. Frequencies were normalized to the activity of each transposable element in JIM17. Error bars represent standard error. Sequences identified at the mPing (5′ TAA/3′ TAA TSDs) excision sites by restriction site analysis and sequencing (b). Underlined sequences indicate the HpaI and HaeIII sites used for analysis. Red bases are unique to the ADE2* template. *indicates the excision site was repaired by HR using the ADE2* template