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Fig. 4 | Mobile DNA

Fig. 4

From: A host factor supports retrotransposition of the TRE5-A population in Dictyostelium cells by suppressing an Argonaute protein

Fig. 4

Expression of TRE5-A in knockout mutants of RNAi components. Expression of TRE5-A ORF1 was analyzed by qRT-PCR in the indicated knockout mutants of Argonaute genes and the Dicer-like protein DrnB. Phenotype reversion in agnC and agnE knockouts was accomplished using TAP-tagged agnC and agnE overexpressed in the respective mutants (agnC [agnC OE] and agnE [ agnE OE]). TRE5-A expression in JH.D cells is shown for comparison. Expression levels were compared to AX2 wild-type cells and are expressed as fold change of expression, meaning that values >1 represent overexpression of genes in the mutants. Data represent means from three independent cultures ± SD. *p < 0.05, **p < 0.01 relative to AX2 cells (Student’s t-test)

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