Figure 1

Phage A118 integration reaction and the subfamily of serine recombinases with large C-terminal domains. (A) Schematic representation of phage A118 integrative recombination, where attP on the phage genome recombines with attB within comK in the Listeria monocytogenes chromosome to form the two junction recombination sites attL and attR. (B) Phylogenetic tree of representative serine recombinases with large C-terminal domains (ClustalW2). (C) Sequence (56 bp) surrounding the A118 attP and attB loci. Palindromic sequences within each att site are denoted with arrows, and identical nucleotides between the two recombination sites are marked with dots. We show in this work that the central GG nucleotides define the crossover sites.