Figure 6
From: Regulation of DNA transposition by CpG methylation and chromatin structure in human cells
CpG-methylated transposon plasmids are associated with condensed chromatin. CpG-methylated or untreated pFP-GTC donor plasmids were transfected into HeLa cells, cross-linked, and precipitated with anti-acetylated histone H3 (anti-AcH3) antibodies indicating euchromatin or anti-trimethylated histone H3 lysine 9 (anti-H3triMeK9) antibodies indicating heterochromatin. DNA was purified and quantified by E. coli transformation (A) or by semi-quantitative PCR after serial dilutions of input DNA (B). anti-AcH3: anti-acetylated histone H3; anti-H3triMeK9: anti-trimethylated histone H3 lysine 9; PCR: polymerase chain reaction.