Figure 6

Transposition frequencies of mini-Tn5 derivatives in isogenic hns + and Δ hns strains. Mating out experiments were carried out on hns + and Δhns strains of E. coli containing a plasmid encoding one of the mini-Tn5 derivatives indicated and a compatible plasmid encoding transposase (M56A) expressed from its native promoter (see Methods for details). Each graph provides transposition frequencies of individual clones (three to five per strain) for at least three and up to four independent experiments. Each experiment compared transposition levels for a pair of isogenic strains containing the same mini-Tn5 substrate. Horizontal and vertical lines are the mean and the standard deviation, respectively, for the transposition frequencies. The fold difference between hns + and Δhns strains for each experiment is indicated in the upper portion of each graph. The t-test to measure variance was used to determine if differences between frequencies within each mating out experiment were/were not due to chance. *P <0.05, **P <0.01, ***P <0.001.