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Table 1 Comparing the qPCR-TI method with other standard techniques

From: Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers

Clone name Methods Copy numbers
   By standard methods By qPCR-TIa
Transposons carrying the neomycin resistance gene
2/1 Transposon display/Southern blotting 8 to 10 8
2/2 Transposon display/Southern blotting 3 4
2/3 Transposon display/Southern blotting 10 to 12 10
2/9 Transposon display/Southern blotting 1 1
1 Transposon display/Southern blotting 12 to 13 13
4 Dot blot 52 50
5 Transposon display/Southern blotting 15 15
6 Transposon display/Southern blotting 12 11
7 Transposon display/Southern blotting 1 1
8 Transposon display/Southern blotting 2 2
9 Transposon display/Southern blotting 1 1
Transposons carrying the amaxaGFP transgene
A3 Splinkerette PCR/inverse PCR 2 2
A4 Splinkerette PCR/inverse PCR 4 4
A5 Splinkerette PCR/inverse PCR 4 4.5b
A6 Splinkerette PCR/inverse PCR 2 2
B1 Splinkerette PCR/inverse PCR 1 2
B3 Splinkerette PCR/inverse PCR 3 3
B5 Splinkerette PCR/inverse PCR 2 2
  1. aQuantitative PCR, transgene independent.
  2. bFor sample A5 from the amaxa green fluorescent clones, real-time PCR measurement indicated that it is more likely to be a mixed population of cells rather than a single clone.