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Table 1 Comparing the qPCR-TI method with other standard techniques

From: Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers

Clone name

Methods

Copy numbers

  

By standard methods

By qPCR-TIa

Transposons carrying the neomycin resistance gene

2/1

Transposon display/Southern blotting

8 to 10

8

2/2

Transposon display/Southern blotting

3

4

2/3

Transposon display/Southern blotting

10 to 12

10

2/9

Transposon display/Southern blotting

1

1

1

Transposon display/Southern blotting

12 to 13

13

4

Dot blot

52

50

5

Transposon display/Southern blotting

15

15

6

Transposon display/Southern blotting

12

11

7

Transposon display/Southern blotting

1

1

8

Transposon display/Southern blotting

2

2

9

Transposon display/Southern blotting

1

1

Transposons carrying the amaxaGFP transgene

A3

Splinkerette PCR/inverse PCR

2

2

A4

Splinkerette PCR/inverse PCR

4

4

A5

Splinkerette PCR/inverse PCR

4

4.5b

A6

Splinkerette PCR/inverse PCR

2

2

B1

Splinkerette PCR/inverse PCR

1

2

B3

Splinkerette PCR/inverse PCR

3

3

B5

Splinkerette PCR/inverse PCR

2

2

  1. aQuantitative PCR, transgene independent.
  2. bFor sample A5 from the amaxa green fluorescent clones, real-time PCR measurement indicated that it is more likely to be a mixed population of cells rather than a single clone.