From: Reliable transgene-independent method for determining Sleeping Beauty transposon copy numbers
Clone name | Methods | Copy numbers | |
---|---|---|---|
By standard methods | By qPCR-TIa | ||
Transposons carrying the neomycin resistance gene | |||
2/1 | Transposon display/Southern blotting | 8 to 10 | 8 |
2/2 | Transposon display/Southern blotting | 3 | 4 |
2/3 | Transposon display/Southern blotting | 10 to 12 | 10 |
2/9 | Transposon display/Southern blotting | 1 | 1 |
1 | Transposon display/Southern blotting | 12 to 13 | 13 |
4 | Dot blot | 52 | 50 |
5 | Transposon display/Southern blotting | 15 | 15 |
6 | Transposon display/Southern blotting | 12 | 11 |
7 | Transposon display/Southern blotting | 1 | 1 |
8 | Transposon display/Southern blotting | 2 | 2 |
9 | Transposon display/Southern blotting | 1 | 1 |
Transposons carrying the amaxaGFP transgene | |||
A3 | Splinkerette PCR/inverse PCR | 2 | 2 |
A4 | Splinkerette PCR/inverse PCR | 4 | 4 |
A5 | Splinkerette PCR/inverse PCR | 4 | 4.5b |
A6 | Splinkerette PCR/inverse PCR | 2 | 2 |
B1 | Splinkerette PCR/inverse PCR | 1 | 2 |
B3 | Splinkerette PCR/inverse PCR | 3 | 3 |
B5 | Splinkerette PCR/inverse PCR | 2 | 2 |