Figure 4
From: Immunity of replicating Mu to self-integration: a novel mechanism employing MuB protein
ChIP reactions probing binding of c-myc-MuB and MuA on the Mu genome during Mu replication. ChIP samples were prepared with either anti-c-myc antibody or anti-MuA antibody using strain SJG3 as described in Methods. Binding on the seven different segments of Mu genome shown in Figure 1a was tested by regular PCR amplification. Input = fragmented whole genome DNA; ChIP = DNA in ChIP samples; Mock = DNA recovered without addition of specific antibody during the ChIP procedure.