Analysis of unique inter-retrotransposon amplified polymorphism (IRAP) bands. In this example, IRAP was conducted with primer 2109, matching the Daniela retrotransposon long terminal repeat (LTR) (Table S2 in Additional file 2). The band was cut and sequenced. Two new primers were designed to match the sequence and the uniqueness of the band was checked on the set of DNA samples. This new insertion is in repetitive DNA (Table S2 in Additional file 2).