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Figure 5 | Mobile DNA

Figure 5

From: Transposition of the Tourist-MITE mPing in yeast: an assay that retains key features of catalysis by the class 2 PIF/Harbinger superfamily

Figure 5

Arabidopsis transposition assay. Comparison of the transposition activity of wild type TPase and the TPase (L384/386A) nuclear export signal mutant in Arabidopsis containing an mPing gfp reporter construct. ORF1 and TPase proteins were expressed from a cDNA that contains both genes [TPase is presumably expressed by a native promoter within the intergenic region] (A). The relative frequency of mPing excision for each construct is shown together with a representative green fluorescent protein (GFP) expressing plant (B). GFP expression produces green sectors on a red chlorophyll background. Polymerase chain reaction analysis of representative GFP expressing plants from each treatment using primers (shown in A) that flank the excision site (C).

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ISSN: 1759-8753