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Table 4 TnsC increases TnsD binding to attTn7 in vivo

From: Characterization of the TnsD-attTn7 complex that promotes site-specific insertion of Tn7

Tns protein

Lysogeny frequencya

TnsC

< 8.0 × 10-7b

TnsD

2.4 × 10-3

TnsC + TnsD

2.4 × 10-1

  1. aUsing the in vivo challenge phage assay, adding TnsC to TnsD increased lysogeny, also indicating that TnsC is stabilizing TnsD on the DNA. TnsC alone did not increase lysogeny.
  2. bLowest possible measurement in this assay.
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Mobile DNA

ISSN: 1759-8753