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Fig. 8 | Mobile DNA

Fig. 8

From: RepEnTools: an automated repeat enrichment analysis package for ChIP-seq data reveals hUHRF1 Tandem-Tudor domain enrichment in young repeats

Fig. 8

RepEnTools reveals enrichment of murine UHRF1 binding on young, species-specific REs with overlapping H3K4me1-K9me3. A Experimental design of Haggerty et al. for endogenous mUHRF1 ChIP-seq in murine ESC [54]. B Genome-wide Pearson correlation r-value comparison of the mUHRF1 profile to various histone H3 marks using 5 kb bins. C RepEnTools analysis reveals mUHRF1 enrichment on LTRs flanking IAP and IAPEz internal sequences. D Confirming RepEnTools’ findings, UHRF1 enrichment is found over the entire length of many IAP-LTRs at regions overlapping the H3K4me1-K9me3 double mark. IAP-LTRs contain experimentally validated enhancer sequences [57, 58]. E UHRF1 is enriched at the 5′ end of IAPEz longer than 5 kb, colocalising with H3K4me1- K9me3, and it was implicated in silencing IAPEz-gag expression [54, 59]. IAPEz are young, mouse-specific ERVKs. ChIP-seq data for mUHRF1 and the histone H3 marks from mESC were published previously [54, 60, 61]

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