Fig. 2

Smarcad1 plays a role in MLV repression. A Smarcad1 and TEs expression changes (shSmarcad1 vs shControl) were measured by RT‒qPCR, normalized to UBC control gene. n = 3–5, data are the mean ± s.e.m (B) Immunoblotting of shSmarcad1 and shControl ESC extract using Smarcad1 antibody, with anti-Gapdh as loading controls (C) The percentage of GFP-positive cells 2 and 7 days after infection by PBSpro or (D) PBSgln virus in the WT and Smarcad1 depleted ESCs., n = 4–6, data are the mean ± s.e.m. E Change in GFP-positive cells after Smarcad1 depletion (F) Change in GFP-positive cells after infection by PBSpro in the. n = 2–7, data are %GFP positive in depleted cells vs. the control cells, values are the mean ± s.e.m. In all panels, P value was calculated using Mann–Whitney U Test:, *P < 0.05, **P < 0.01, ***P < 0.001