Fig. 1

Smarcad1 is recruited to newly integrated retroviruses. ChIP‒qPCR was performed using Smarcad1 antibody, 2 days after MLV-PBSpro infection. Values shown are mean ± s.e.m, relative to the total input samples and normalized to the signal of negative control gene (Gapdh). The illustration above the graph indicates the position of the primers on the MLV vector: 40 nt is located by the NCR, at the 5’ end of the LTR, next are the TIS [transcription initiation site], PBS [primer-binding site], and GFP [Green fluorescence protein] primers. n = 2, Data are the mean ± s.e.m, asterisk shows significant difference from the negative control genes, P value was calculated using Mann–Whitney U Test, *P < 0.05, **P < 0.01