Fig. 2

IFNAR1.L1M2a was chosen as an example locus for experimental exploration. A IFNAR1.L1M2a (blue) contains a predicted distal enhancer element identified by the ENCODE consortium (orange) [43]. Within or nearby this predicted enhancer region are several immune-related transcription factor binding motifs (red, purple). We sought to knock out this potential enhancer region, dubbed IFNAR1.L1M2a.enh (black). B The IFNAR1 gene (navy) locus. Publicly available ChIP-seq data (red), obtained from the Blueprint Database [28] and ENCODE consortium [29], along with our CUT&Tag data, describing the epigenetic landscape at the IFNAR1.L1M2a locus. Naive B cells, class switched memory B cells and the B cell-like GM12878 cell line exhibit bivalent repressive and enhancer associated histone modifications across the L1M2a insertion (highlighted blue). PRO-seq (purple) and ATAC-seq (green) both support the presence of an enhancer at the predicted site in IFNAR1.L1M2a, potentially induced by type I IFN treatment