Fig. 1
RNA-seq data obtained from B. hordei-infected barley leaf epidermal peelings exhibits time point-dependent clustering. A We sampled B. hordei isolate K1AC-infected barley abaxial leaf epidermis at time points across the asexual life cycle of the fungus. The pictograms indicate the fungal stage from conidiospore germination (0 hpi) to conidiogenesis, i.e., asexual spore formation (120 hpi). B and C Left panels: Horizontal clustering using the normalized gene expression values of B. hordei isolate K1AC (B) and H. vulgare cv. ‘Margret’ (C), respectively, was calculated with Euclidean distance and Ward.D2 clustering in R. Height indicates the Euclidean distance between samples. Middle panels: Principal components were computed using the R algorithm prcomp. The percentage of sample divergence explained by the principal components PC1 and PC2 are indicated as ratios in the axis labels. Right panels: Non-metric multidimensional scaling (NMDS). Colors: Burgundy, 0 hpi; red, 6 hpi; yellow, 18 hpi; light green, 24 hpi; blue, 72 hpi; purple, 120 hpi