The unusual structure of the PiggyMac cysteine-rich domain reveals zinc finger diversity in PiggyBac-related transposases

Table 1 NMR and refinement statistics for Pgm(692-768)* structures generated with CYANA

Experimental restraints
Total number of restraints	1066
Intra-residual and sequential	736
Medium range (1 < \|i_j\| < 5)	108
Long range	222
TALOS+ Dihedral angles	116
CYANA target function	0.55 ± 0.09
Rmsd from experimental restraints^a
Upper limits (Å)	0.006 ± 0.003
Dihedral angles (deg)	0.392 ± 0.094
Rmsd from idealized geometry^a
Bonds (Å)	0.0012 ± 0.0008
Angles (deg)	0.53 ± 0.50
Rmsd from mean structure^a
Backbone atoms (Å)	0.54 ± 0.19 Å
Heavy atoms (Å)	1.03 ± 0.19 Å
Ramachandran plot^b
Most favored	90.0%
Additionally allowed	10.0%
Generously allowed	0.0%
Disallowed	0.0%

^aCalculated over residues 701-749 of the 15 selected structures
^bCalculated over ordered residues 698-714, 717-750, 762-764 of the 15 selected structures

ISSN: 1759-8753