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Table 2 Transposase-mediated germline integration

From: Active recombinant Tol2 transposase for gene transfer and gene discovery applications

Site

Sequence

Chr.

Refseq

1

aaatatttaccaagcaac-5′-Tol2-3′-caagcaacacgttcagtg

8

Intron of gfra2

2

ataatttcctcttatttg-5′-Tol2-3′-cttatttgcatgtcagat

13

Intergenic

3

cgcatgctaacttataga-5′-Tol2-3′-cttatagaggaggtgccc

8

Exon of wu: fb79a07

4

aaacgttcctcctaacac-5′-Tol2-3′-cctaacacagttagatgg

3

Intergenic

5

caacacatgactcgttgg-5′-Tol2-3′-ctcgttggccatatgcta

15

Intergenic

6

gggaatatgtgttattaa-5′-Tol2-3′-gttattaactgcgtccca

4

Repetitive sequences

7

agctgtctcttctgtgtc-5′-Tol2-3′-tctgtgtcattcagtctc

3

Intron of LOC557901

8

tgtcagagatctaggtca-5′-Tol2-3′-ctaggtcagatggaggaa

25

Intergenic

  1. Tol2 insertion sites generated by His-Tol2 protein co-injection were cloned from F1 transgenic fish. Examples of 8-bp genomic sequence duplication (in bold) were shown with insertion loci information