Skip to main content

Table 1 His-Tol2 and Tol2 mRNA as sources of transposase in vivo

From: Active recombinant Tol2 transposase for gene transfer and gene discovery applications

Somatic F0 pattern

Tol2 form

Germline trapping and expression frequency

Average F0 germline mosaicism

Cat II.

mRNA

65 % (13/20)

27 %

Cat III.

mRNA

88 % (35/40)

39 %

Cat III.

Protein

76 % (13/17)

39 %

Cat IV.

Protein

90 % (9/10)

51 %

  1. Analyses of somatic and germline transmission of reporter genes are shown. Fish injected with either mRNA or His-Tol2 were characterized by different F0 somatic patterns and assessed for corresponding germline trapping frequency, estimated as the percentage of F0 fish producing GFP-positive offspring. The number of fish screened is shown in parentheses. The F0 germline mosaicism rate was determined by the percentage of GFP-positive F1 offspring from a founder outcross. The average mosaicism rate for founder fish is listed from each different category
Back to article page

Mobile DNA

ISSN: 1759-8753