Fig. 6

Comparison of predicted ZFP809 target motifs and experimentally identified target sequence of ZFP809. a The ZFP809 protein sequence was used to infer predicted target motifs using an expanded linear support vector machine (SVM) (top) or a polynominal SVM (middle) [27] and a prediction tool based on bacterial one-hybrid screens and ChIP-seq data (bottom) [9]. The canonical PBS^pro sequence is shown below. b One of the predicted motifs [9] was used to screen matching genomic sites of the mouse genome using the software tool FIMO [199]. The 500 top-scored sites and the 149 genomic PBS^pro were screened for ZFP809 enrichment by NGS.plot [200] and published FLAG-ZFP809 ChIP-seq data in murine embryonic carcinoma cells [7]