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Figure 3 | Mobile DNA

Figure 3

From: Distinctive patterns of epigenetic marks are associated with promoter regions of mouse LINE-1 and LTR retrotransposons

Figure 3

Co-occupancy of histone marks at the interspersed nuclear element-1 (L1) and long terminal repeat (LTR) promoters. Sequential chromatin immunoprecipitation (SeqChIP) was performed on chromatin from L929 (A) and NIH3T3 (B) cells using H2A and H2A.Z antibodies, followed by the second ChIP reactions as described above. The resulting enriched DNA was probed for LINE-1, LTR (mouse endogenous retrovirus (Mm ERV) and intracisternal A-particle (IAP)), and SINE-B1 using consensus primers by qPCR. The relative enrichment was calculated as the percentage of the first ChIP for H2A or H2A.Z-containing DNA that also contains second ChIP DNA. Percent occupancy above 50% was considered as significant. Error bars, s.d. of three biological replicates.

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ISSN: 1759-8753