Figure 2From: Characterization of a synthetic human LINE-1 retrotransposon ORFeus-HsTotal RNA analysis of L1 expression. Expression levels of native, partially synthetic, and completely synthetic ORFeus-Hs were compared in 293T cells. The vectors used were: pLD223, pWA172, pWA170, pWA176, pWA163, pWA174, pLD224, pLD227, pLD225, pWA165. Top, L1 mRNA expression; note both spliced and unspliced transcripts. Bottom, RNA expression of loading control, ARPP0.Back to article page