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Table 2 Interactions of Gag3 mutants

From: Two-hybrid analysis of Ty3 capsid subdomain interactions

 

V

Gag3

D60A/R63A

MHR2

MHR4

E148A/K149A

E190A/R191A

V

-

-

-

-

-

-

-

Gag3

-

+++

-

-

-

-

+++

D60A/R63A

-

+

-

-

-

-

++

MHR2

ND

++

-

-

-

-

++

MHR4

ND

++

-

-

-

-

++

E148A/K149A

-

++

-

-

-

-

++

E190A/R191A

-

+++

-

-

-

-

+++

  1. Top row, Gal4-BD fusions; first column, Gal4-AD fusions; V, first row = pGBK vector; V, first column = pGAD vector; AD = activation domain; BD = binding domain; CTD = C-terminal domain; NTD = N-terminal domain; ND = not determined.
  2. For images, see Additional files 1, 2, 3, 4, 5, 6.