Fig. 5

RT-sensitive interferon-inducible genes and IFNB in neutrophils and lymphocytes from SLE patients and healthy volunteers. A Real-time PCR quantitation of four representative interferon-stimulated genes (ISGs) from neutrophils from SLE patients (n = 4) or HC (n = 1). The data represent expression normalized by GAPDH signal. B Real-time PCR quantitation of the same ISGs from mononuclear cells from SLE patients (n = 4) or HC (n = 1). The data represent expression normalized by GAPDH signal. C Composite scores (mean) of the same ISGs from neutrophils from SLE patients (n = 14) or HC (n = 7). D Increased expression of one hundred and eight ISGs identified by RNA-Seq in SLE neutrophils (SLE-PMN) relative to healthy donor neutrophils and in SLE mononuclear cells (SLE-PBMC) relative to healthy donor mononuclear cells. The data points represent the average of each gene among the patients relative to the average expression of the same gene in healthy controls. E Real-time PCR quantitation of the ISGs and IFNB from neutrophils kept in cell culture for 4 h without or with 10 µM emtricitabine and 1.25 µM tenofovir alafenamide. The data represent the average of 3 independent experiments. F The same data as in E shown as individual patients (n = 3) and as percent of control (100% = without inhibitors)