Fig. 3

CK2 holoenzyme inhibits Ty1 retrotransposition. A Retrotransposition frequencies (log scale, mean ± SD, n ≥ 3) of a chromosomal Ty1-his3AI reporter in WT cells and non-essential mutants of the CK2 complex. Welch’s t-test with comparison to the WT strain: ns, not significant; *p < 0.05; **p < 0.01, ***p < 0.001. B Detection by PCR of de novo insertions of endogenous Ty1 elements upstream of the Pol III-transcribed gene SUF16 and at the HXT subtelomeric loci in the indicated strains using a primer in Ty1 (red triangle) and a primer in the locus of interest (blue triangle). Total genomic DNA was extracted from His⁺ cells obtained from 4 independent cultures. Two different exposures are displayed for the SUF16 locus to clearly show the increase in insertions in the cka2∆ ckb2∆ mutant compared to the other strains. C Genome-wide Ty1-HIS3 insertions. Percentage of insertions that occurred 1 kb-window upstream of Pol III-transcribed genes, in verified ORFs, subtelomeres, telomeres, retrotransposons, ARS and random insertions generated in silico. D Retrotransposition frequencies (log scale, mean ± SD, n ≥ 3) of a pPSP2-Ty1-his3AI reporter carried on a centromeric plasmid in the indicated cells. Welch’s t-test with comparison to the WT strain is indicated above each bar. Welch’s t-test with comparison between WT pPSP2-Ty1-his3AI in ste12∆ and ste12∆ cka2∆ ckb2∆ strains is indicated by a bracket. ns, not significant; *p