Skip to main content
Fig. 6 | Mobile DNA

Fig. 6

From: Mobile group I introns at nuclear rDNA position L2066 harbor sense and antisense homing endonuclease genes intervened by spliceosomal introns

Fig. 6

In vivo maturation of I-DsqI mRNA in Didymium squamulosum. a Schematic presentation of I-DsqI mRNA. Start (AUG) and stop (UAA) codons are indicated, as well as the 47 nt spliceosomal intron (I-47). Two AAUAAA polyadenylation signals (I and II) are located close to the termination codon. Primer locations used in assessment of intron removal and polyadenylation are indicated. b RNA from amoeba cells was analyzed by an RT-PCR approach using a primer set flanking I-47. Amplicons corresponding to I-47 lacking (−) and I-47 containing (+) mRNAs were detected by gel analysis (left) and confirmed by Sanger sequencing (right). c RNA from amoeba cells was analyzed by an RT-PCR approach using a primer set flanking the polyadenylation signals. A poly(T) specific primer was used in the first-strand synthesis reaction that contained a unique sequence tag at the 5′ end, corresponding to a tag-complementary downstream primer. Amplicons containing polyA tails were detected by gel analysis (left) and confirmed by Sanger sequencing (right)

Back to article page