Fig. 2

L1 mRNA expression is consistent in MCF7 single cell clusters. A. The number of million mapped reads per cell is shown for each tSNE cluster of MCF7 cells (ANOVA, ****,< 0.0001). B. The L1 expression level per cell quantified by RPM is shown for Clusters 1, 3, and 4 in the Violin Plot (ANOVA, ****,< 0.0001). C. The number of expressed L1 loci per cell is shown for MCF7 clusters 1, 3, and 4 (ANOVA, *, < 0.05). D. The number of expressed L1 loci shared between MCF7 clusters 1, 3, and 4 is shown in the Venn diagram. E. The L1 FPKM values for bulk RNA-Seq (left y-axis) and L1 RPM values for High coverage scRNA-Seq (right y-axis) of MCF7 cells are shown in the dot-plot. Orange circles indicate L1 loci with expression detected in the bulk dataset that were not detected to be expressed in the scRNA-Seq dataset. The nested Venn diagram shows the number of shared expressed L1 loci from the bulk and scRNA-Seq datasets. The violin plot shows the FPKM values for expressed L1 loci unique to the bulk dataset and those shared between bulk and scRNA-Seq (Welch’s t-test, ****,< 0.0001). F. The number of expressed L1 loci validated with bulk RNA-Seq is shown in darker colors with the number of expressed L1 loci without bulk RNA-Seq confirmation shown in lighter colors. G. The L1 RPM levels per MCF7 cluster for L1 loci confirmed with bulk RNA-Seq are shown in dark colors with the RPM levels for L1 loci with no bulk RNA-Seq confirmation shown in light colors