Fig. 4

Contribution of different lengths of monomer 3 to overall promoter activity in NIH/3T3 cells. A Distribution of 5’UTR start positions for 157 Tf_I loci that are 5’ truncated within M3. The x-axis represents nucleotide positions from 1 to 212 for M3. The y-axis displays the count of Tf_I loci that start at each nucleotide position. The three most frequent nucleotide positions are annotated. B Distribution of 5’UTR start positions for 357 A_I loci that are 5’ truncated within M3. C Normalized promoter activity of Tf_I 5’UTR consensus sequences with varying M3 length. Sequence organization of the promoters is illustrated on the left side. The length of M2, M1, and T for each promoter is annotated (in base pairs). The x-axis indicates the normalized promoter activity. Note promoter constructs in panel C were tested together in the same 96-well plate with those for Fig. 3A; thus, L1_RP, M3-M2-M1-T and M2-M1-T are shared between Fig. 3A and Fig. 4C. D Normalized promoter activity of A_I 5’UTR consensus sequences with varying M3 length. Note promoter constructs in panel D were tested together with those for Fig. 3C